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Polyamides 12-(R,S) and 12-(R,R) are separable by UPLC. An overlay of three separate UPLC runs (λ = 310 nm) is shown. A 2:1 ratio of 12-(R,R):12-(R,S) was utilized to optimize separation and to assign retention times to diastereomers. Individual runs of 12-(R,R) and 12-(R,S) each revealed 99% de by integration of the area of the UV–vis signal (λ = 310 nm).
