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. 2012 May 16;32(20):6967–6980. doi: 10.1523/JNEUROSCI.0025-12.2012

Figure 1.

Figure 1.

Effect of S-SCAM overexpression on the dendritic spines of hippocampal neurons. Hippocampal neurons were cotransfected with S-SCAM (myc-tagged) and β-gal or GFP, and their dendritic spines were analyzed. A, Representative images of dendrites from hippocampal neurons cotransfected with S-SCAM (red) and β-Gal (green), showing synaptic localization of S-SCAM. Scale bar, 5 μm. B, Effect of S-SCAM overexpression on dendritic spine density. Representative images of dendrites from neurons transfected with β-Gal alone (β-Gal, top) and S-SCAM + β-Gal (S-SCAM, bottom) are shown on the left and quantified spine density data are on the right. n > 20 per condition. ***p < 0.001. C, Representative images of dendritic spines from control (GFP only) or S-SCAM-transfected neurons. Vertical and horizontal dotted lines represent the length and width of dendritic spines, respectively. D, E, Effect of S-SCAM overexpression on the width (D) and length (E) of dendritic spines. F, G, Cumulative plot analyses of dendritic spine width (F) and length (G). p < 0.001 for width and p = 0.35 for length (K-S test). H, Changes in the morphology of dendritic spines after S-SCAM overexpression. Dendritic spines from >10 neurons per group were analyzed.