Table 4.
Global DNA methylation in tissues from ApoE mice fed a control (C) or high fat (HF) diet depleted of folic acid (−F) or folic acid and vitamins B6 and B12 (−F−B) for 16 weeks
| Global DNA methylation | Control fat | High fat | |||||
|---|---|---|---|---|---|---|---|
| C | C−F | C−F−B | HF | HF−F | HF−F−B | ||
| Aorta | 5-methyldeoxycytidine (ng/μg DNA) | 125.5 ± 8.2 (6) | 135.7 ± 7.7 (6) | 134.4 ± 22. (6) | 116.6 ± 11.5 (6) | 123.7 ± 7.8 (6) | 133.2 ± 15.7 (6) |
| DNA methylation (%) | 3.72 ± 0.06 (6) | 3.61 ± 0.05 (6) | 3.62 ± 0.07 (6) | 3.60 ± 0.05 (6) | 3.57 ± 0.04 (6) | 3.58 ± 0.07 (6) | |
| Heart | 5-methyldeoxycytidine (ng/μg DNA) | 158.9 ± 10.9 (6) | 135.3 ± 4.8 (6) | 130.8 ± 3.7 (6) | 134.7 ± 6.1 (6) | 157.0 ± 13.4 (6) | 148.8 ± 9.8 (6) |
| DNA methylation (%) | 3.8 ± 0.08 (6) | 3.68 ± 0.11 (6) | 3.75 ± 0.04 (6) | 3.79 ± 0.05 (6) | 3.85 ± 0.12 (6) | 3.81 ± 0.05 (6) | |
| Aorta tunica adventitia | 5-methyldeoxycytidine (ng/μg DNA) | 137.9 ± 12.2 (9) | 141.0 ± 8.6 (10) | 184.9 ± 22.3 (10) | 171.5 ± 13.0 (8) | 149.7 ± 20.1 (9) | 157.8 ± 16.2 (7) |
| DNA methylation (%) | 4.05 ± 0.11 (10) | 3.94 ± 0.15 (10) | 4.25 ± 0.10 (10) | 4.31 ± 0.12 (8) | 3.99 ± 0.10 (9) | 3.98 ± 0.13 (7) | |
| Liver | 5-methyldeoxycytidine (ng/μg DNA) | 68.1 ± 5.5 (10) | 66.6 ± 4.7 (10) | 71.4 ± 5.3 (10) | 71.5 ± 5.9 (10) | 68.4 ± 6.5 (10) | 67.6 ± 6.6 (10) |
| DNA methylation (%) | 3.19 ± 0.04 (10) | 3.20 ± 0.04 (10) | 3.21 ± 0.05 (10) | 3.21 ± 0.05 (10) | 3.20 ± 0.06 (10) | 3.21 ± 0.05 (10) | |
Values are means ± SEM with the number of mice sampled per group in parentheses