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. 2011 May 24;21(11):1564–1577. doi: 10.1038/cr.2011.84

Figure 3.

Figure 3

Presence of neutrophils in the alveolar compartment. (A) Immunohistochemistry (IHC) of paraffin-embedded lung sections from WT (upper panels) and KO (lower panels) mice at postnatal day 7, comparing levels of neutrophil alveolar infiltration by the specific reactivity of monoclonal antibody MCA771GA (right panels). Staining with isotype-matched control IgG (left panels) was also performed to validate MCA771GA antibody specificity. (B) Quantitative measurements of MCA771GA+ alveolar infiltrates performed by double-blind counts of three independent readings of five distinct IHC fields. Bars indicate ± SEM (n = 15, P < 0.05).