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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Lab Invest. 2012 Mar 26;92(6):827–841. doi: 10.1038/labinvest.2012.53

Figure 3. Curcumin abrogated the effect of AGEs and induced gene expression of AGE-R1 in cultured HSCs.

Figure 3

Serum-starved HSCs were treated with AGEs at 0–200 μg/ml (A & B), or with AGEs at 100 μg/ml plus curcumin at indicated concentrations (D & E), in serum-depleted media for 24 hr. On the other hand, passaged HSCs were treated with curcumin at indicated concentrations in DMEM with 10% FBS for 24 hr (C).

A & D: Real-time PCR analyses. Values were presented as mRNA fold changes (mean ± s. d., n=3). *p<0.05 vs. the untreated control (the 1st column), ‡p<0.05 vs. the cells treated with AGEs alone (the 2nd column).

B, C, & E: Western blotting analyses. Representatives were from three independent experiments. β-tubulin was used as an internal control for equal loading. Italic numbers beneath blots were fold changes (means ± s. d.) in the densities of the bands compared with the control without treatment in the blot (n=3), after normalization with the internal invariable control.