Table 3.
Phosphorylation motifs unique to HAP-MudPIT and HILIC-IMAC-LC-MS/MS with fold increases greater than 5.
| HAP-MudPIT motif | Fold increase | HILIC-IMAC motif | Fold increase |
|---|---|---|---|
| ……s.DDE.. | 64.80 | ..P.SPt…… | 26.35 |
| ……s.DEE.. | 47.25 | …..PsTP…. | 22.44 |
| ……tD.E… | 35.75 | ……sEEE… | 13.80 |
| ……s.D.D.. | 16.45 | …..PsSP…. | 12.25 |
| ……sE.D… | 15.38 | ……tP.K… | 10.16 |
| …D..sD….. | 13.49 | ….SDsE….. | 9.99 |
| ……S.D..D. | 13.24 | ……sP.K… | 8.63 |
| …..Ds…E.. | 12.52 | ……sPT…. | 8.35 |
| ……s…E.D | 12.29 | ……sPK…. | 8.2 |
| ……S…D.D | 11.97 | ……tP.P… | 7.84 |
| ……S.E..D. | 9.52 | ….S.tP….. | 7.81 |
| ……S E.E | 9.37 | ……SP…R. | 7.7 |
| ……sP..E.. | 9.27 | ……t.SP… | 6.59 |
| ……S.E..E. | 8.09 | .R.R..s…… | 6.46 |
| ……sPE…. | 7.99 | ……sPS…. | 6.03 |
| ..E…S..E… | 7.64 | ….R.tS….. | 6.03 |
| Average | 18.44 | …SP.t…… | 5.55 |
| Count | 16 | ……s.TP… | 5.11 |
| Average | 9.96 | ||
| Count | 18 | ||
Motifs and fold increases were found using Motif-X.86 All motifs and fold increases represented in Figure 4 can be found in the Supporting Material.