Skip to main content
. 2011 Mar 24;2(3):e2. doi: 10.1038/ctg.2011.1

Figure 3.

Figure 3

Colonic cytokine and chemokine profiles. The cytokine expression profile upon oral delivery of cyclin D1 (CyD1), tumor necrosis factor-α (TNF-α), or a combination of both short interfering RNA (siRNA) encapsulated in nanoparticles-in-microsphere oral system (NiMOS) was determined using a chemiluminescent enzyme-linked immunosorbent assay Q-Plex Mouse Cytokine Screen (Quansys Biosciences). Concentrations of the cytokines (a) interferon (IFN)-γ, (b) interleukin (IL)-1α, (c) IL-1β, (d) IL-2, (e) IL-5, (f) IL-6, (g) IL-17 and pro-inflammatory chemokines (h) monocyte chemotactic protein (MCP)-1, (i) monocyte inflammatory protein (MIP)-1α, and (j) granulocyte macrophage colony-stimulating factor (GMCSF) in the large intestine are shown. Administration of CyD1 NiMOS led to a significant reduction in protein expression both time points tested compared with the remaining groups. The effect of combined TNF-α/CyD1 NiMOS and TNF-α was less pronounced, but led to decreased protein levels in comparison with control groups. Values expressed as mean±s.d. (n=5). ΔP<0.05, vs. DSS control; ΔΔP<0.01, vs. DSS control; *P<0.05, vs. Scramble; **P<0.01, vs. Scramble; Statistical comparison was performed on data sets of DSS control vs. TNF-α, Cyclin D1, and TNF-α/Cyclin D1 combination NiMOS, and between TNF-α, Cyclin D1, and TNF-α/Cyclin D1 combination vs. Scramble NiMOS group. Only significant differences are shown.