FIGURE 5.

Enhanced levels of phospho-IP₃R and calpains in CNGA3^−/−/Nrl^−/− and CNGB3^−/−/Nrl^−/− retinas. The expression levels of phospho-IP₃R, calpain I, and calpain II were examined in CNGA3^−/−/Nrl^−/−, CNGB3^−/−/Nrl^−/−, and Nrl^−/− mice at P30. A, shown are representative images of the Western blot detections. Total retinal protein lysate was used for the detections, and actin was used as loading control. B, densitometric analysis of the relative expression levels of phospho-IP₃R (left panel) and calpain II (right panel) in CNGA3^−/−/Nrl^−/−, CNGB3^−/−/Nrl^−/−, and Nrl^−/− retinas. Data are represented as means ± S.E. of measurements from three to four independent experiments using retinas from four to five mice. Unpaired Student's t test was used for determination of the significance (*, p < 0.05). C, shown are representative images of the Western blot detections of Bcl-2 and Bcl-x in the mouse retina. Total retinal protein lysate was used, and actin was used as a loading control.