FIGURE 7.

Expression of mitochondrial apoptotic proteins in CNGA3^−/−/Nrl^−/− and CNGB3^−/−/Nrl^−/− retinas. The expression levels of the mitochondrial proteins AIF and Endo G were examined in CNGA3^−/−/Nrl^−/−, CNGB3^−/−/Nrl^−/−, and Nrl^−/− mice at P30. A, shown are representative images of the Western blot detections of AIF and Endo G. Retinal nuclear fractions were used for this examination, and H3 was used as a loading control. B, densitometric analysis of the relative levels of AIF and Endo G in the CNGA3^−/−/Nrl^−/−, CNGB3^−/−/Nrl^−/−, and Nrl^−/− retina. Data are represented as means ± S.E. of measurements from three independent experiments using retinas from four to five mice. Unpaired Student's t test was used for determination of the significance (*, p < 0.05). IB, immunoblot.