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Representative titration curves for 15N-labeled distal (A) and proximal (B) Ubs in Lys63-Ub2 and distal (D) and proximal (E) Ubs in Lys48-Ub2 as a function of the molar ratio of VHS-UIM, and for 15N-VHS-UIM as a function of the molar ratio of Lys63-Ub2 (C) and Lys48-Ub2 (F).
