TABLE 1.
Summary of the dissociation constants derived from NMR titration curves for the UIM/mono-Ub and UIM/Ub2 binding equilibria
Kd values are averaged over several residues that showed strong CSPs upon binding. Standard deviations are shown in parentheses.
| Sample | Domain analyzed | Kd |
|---|---|---|
| μm | ||
| UIM/mono-Ub | UIM | 266 (35)a |
| Mono-Ub | 309 (37)a | |
| UIM/Lys48-Ub2 | Distal Ub | 304 (32)b |
| Proximal Ub | 325 (36)b | |
| UIM | 345 (28)b | |
| UIM/Lys63-Ub2 | Distal Ub | 310 (44)b |
| Proximal Ub | 334 (30)b | |
| UIM | 267 (40)b |
a Kd obtained using a 1:1 binding model.
b Kd obtained using a 2:1 binding model.