TABLE 1.

Plasma amino acid levels of WT and Goat^−/− mice at 9:00 a.m. and 5:30 p.m. on day 7 of calorie restriction

Male WT and Goat^−/− littermates (8 weeks old) were subjected to 60% calorie restriction for 7 days. At 9:00 a.m. and 5:30 p.m. on day 7 of calorie restriction, the mice were euthanized, and blood was drawn from each mouse. Plasma amino acid levels were determined by a Hitachi L-8900 amino acid analyzer. Each value represents the mean ± S.E. of data from six mice. The footnotes denote the levels of statistical significance (Student's t test) between WT and Goat^−/− mice at 5:30 p.m. There was no statistical difference between WT and Goat^−/− mice at 9:00 a.m. Dashes indicate that the amino acid levels were undetectable.

Amino acid	9:00 a.m.		5:30 p.m.
	WT	Goat−/−	WT	Goat−/−
	μm		μm
Gly	127 ± 13	120 ± 17	93 ± 14	141 ± 33
Ala	137 ± 15	157 ± 51	72 ± 9.2	243 ± 89a
Ser	58 ± 5.0	52 ± 7.5	44 ± 5.6	107 ± 30a
Thr	70 ± 3.5	67 ± 9.1	60 ± 7.2	182 ± 46a
Cys	11 ± 1.0	10 ± 2.2	4.2 ± 1.4	7.2 ± 1.2
Met	16 ± 0.8	13 ± 1.3	11 ± 2.5	27 ± 8.0
Val	98 ± 9.3	91 ± 8.7	104 ± 27	400 ± 100b
Iso	38 ± 5.3	32 ± 2.0	31 ± 6.7	56 ± 10a
Leu	124 ± 15	94 ± 14	87 ± 42	554 ± 147b
Asn	—	—	—	—
Gln	202 ± 10	183 ± 20	173 ± 17	279 ± 54
Tyr	126 ± 4.9	130 ± 7.8	122 ± 14	181 ± 30
Phe	42 ± 2.8	30 ± 3.3	37 ± 3.5	55 ± 9.9
Trp	4.6 ± 1.3	6.1 ± 1.0	8.8 ± 2.0	12.1 ± 1.0
Lys	172 ± 6.2	144 ± 17	101 ± 25	508 ± 152a
His	20 ± 0.9	15 ± 3.6	18 ± 3.1	70 ± 24a
Arg	41 ± 4.6	22 ± 3.4	20 ± 5.5	37 ± 8.1a
Asp	1.7 ± 0.4	1.7 ± 0.2	3.7 ± 0.7	3.5 ± 0.9
Glu	12 ± 1.9	13 ± 1.5	9.1 ± 1.8	15 ± 3.6
Pro	179 ± 21	142 ± 23	214 ± 86	275 ± 93

^a p < 0.05.

^b p < 0.01.