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. 2012 Apr 9;287(22):18308–18317. doi: 10.1074/jbc.M112.346270

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Key role of BCL6 in thymus B cell expansion of Patz1 knock-out mice. A, immunohistochemical staining of BCL6 in representative thymus samples from Patz1^+/+ (left panel) and Patz1^+/− (right panel) mice. Scale bar, 100 μm. B, Western blot analysis for BCL6 expression in a pool of three Patz1^+/+ and three Patz1^+/− thymi. C, flow cytometry of representative thymi from Patz1^+/+;Bcl6^+/+, Patz1^+/−;Bcl6^+/+, and Patz1^+/−;Bcl6^+/− mice. The thymocytes were double-stained for specific B (CD19) and T cell (CD5) surface antigens. For each analysis, 10,000 events were counted. The relative percentage of CD19⁺ cells (B lymphocytes) was indicated in the right-bottom corner of each dot plot. CD19-PE, CD19-Phycoerythrin staining. D, immunohistochemical analysis of the thymi shown in C to detect B cells (stained with anti-B220). Only scattered positive cells were detected in Patz1^+/+;Bcl6^+/+ (α,β) and most Patz1^+/−;Bcl6^+/− thymi (η,θ). Large focal hyperplasias of B cells were detected in Patz1^+/−;Bcl6^+/+ (γ, δ) thymi, and small focal positivity was detected in some Patz1^+/−;Bcl6^+/− thymi (ϵ, ζ). Scale bars, 100 μm.