FIGURE 2.

Mitochondrial Ca²⁺ uptake in permeabilized cells and mitochondrial membrane potential are not affected by wt α-synuclein overexpression. A, shown are representative traces (left) and average plateau [Ca²⁺]_m values (right, results are the mean ± S.E.) reached in permeabilized cells exposed to 1 μm Ca²⁺-buffered solution. Where indicated, the medium was switched from IB/EGTA-ATP to IB/1 μm Ca²⁺. The traces are representative of at least 14 independent experiments. B, shown is immunocytochemistry analysis on permeabilized HeLa cells overexpressing wt α-syn. The staining with monoclonal antibody against α-syn was revealed by AlexaFluor 488-conjugated antibody. C, HeLa cells (control and overexpressing wt α-syn) were loaded with TMRM probe to determine the mitochondrial membrane potential (ΔΨ). Bars represent the average TMRM fluorescence signals subtracted of signals remaining after FCCP treatment to collapse ΔΨ and are expressed as % Δ fluorescence. The analysis was performed on n = 124 mock cells and on n = 209 α-syn-overexpressing cells.