FIGURE 2.

GyrA and CTD topology footprint assays. A, nicked pSG483 (6 nm) was incubated with varying amounts of the E. coli GyrA CTD (indicated as a molar excess of CTD over plasmid) and ligase. B, nicked pSG483 (6 nm) was incubated with varying amounts of E. coli GyrA dimer (indicated as a molar excess of GyrA dimer over plasmid) and ligase. The positions of relaxed and negatively supercoiled DNA species are labeled with graphic representations on the right.