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Gyrase holoenzyme topology footprint assays. Relaxed pSG483 (6 nm) was incubated with varying amounts of reconstituted holoenzyme (Holo) and topo IB in the absence (top panel) and presence (bottom panel) of AMP-PNP (2 mm) (indicated as a molar excess of holoenzyme over plasmid). The positions of relaxed and negatively supercoiled DNA species are labeled with graphic representations on the right.
