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. 2012 Apr 4;287(22):18500–18509. doi: 10.1074/jbc.M111.327528

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Down-regulation of CTIF triggers the redistribution of CBP80 from polysome fractions to subpolysome fractions. A and B, polysome fractionation analysis of HEK293FT cells either undepleted (A) or depleted of endogenous CTIF (B). Each fraction was subjected to Western blotting using the indicated antibodies. C and D, quantitative representations showing the relative distributions of CBP80 (C) and eIF4E (D) in polysome fractions. The relative levels of CBP80 (C) and eIF4E (D) in each fraction were calculated as a percentage of the total.