Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Reductive nitrosylation observed rate constants for isolated Hb chains. Ferric Hb (HbFe3+) chains were mixed with NO (final concentrations 2–1000 μm) from ProliNONOate. Protein concentrations used (in heme) were 10–45 μm (α-chains) and 10–90 μm (β-chains). Reactions were performed at 37 °C in 100 mm sodium phosphate (pH 7.4). A, Hb α-chains. B, Hb β-chains. The insets show the observed rate constants in the low NO range.
