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Reductive nitrosylation observed rate constants for NEM-modified β-chains and HbA. Ferric Hb (HbFe3+) chains were mixed with NO (final concentrations 2–1000 μm) from ProliNONOate. Protein concentrations used (in heme) were 7–20 μm (NEM-modified β-chains) and 25–50 μm (NEM-modified HbA). Reactions were performed at 37 °C in 100 mm sodium phosphate (pH 7.4). A, NEM-modified HbA; B, NEM-modified β-chains. The insets show the observed rates in the low NO range.
