FIGURE 5.

Effect of IHP on the reductive nitrosylation observed rate contants. We observe that the effect of IHP is small at 37 °C but becomes more noticeable at lower temperatures. A, observed rates of HbFe²⁺-NO formation when HbFe³⁺ (≈100 μm) was mixed with 100 μm NO from ProliNONOate at 15, 25, or 37 °C in the presence of 5 mm IHP. B, observed rate constants of HbFe²⁺-NO formation when HbFe³⁺ (≈100 μm) was mixed with 50 μm NO from ProliNONOate at 15, 25, or 37 °C in the presence of 5 mm IHP.