FIGURE 8.

The presence of small amounts of methemoglobin greatly reduces the stability of NO in solution. A, time course of injections of 125 nm ProliNO (in PBS) (pH 7.4), in the absence or presence of 25 μm methemoglobin into PBS in a purge vessel of NOA. The data are representative of three separate experiments. B, half-life of increasing concentrations of ProliNO (in PBS) (pH 7.4) injected into PBS in a purge vessel of NOA in the absence or presence of 25 μm methemoglobin. Data shown are from the average of three independent experiments.