FIGURE 2.

Tomato LAP-A and LAP-N protect CS from thermal aggregation. A, CS (300 nm) was incubated in 50 mm HEPES-KOH (pH 7.5), 5% glycerol, and His₆-LAP-A or lysozyme (1200 nm) (♢) at 43 °C for 60 min. The LAP-A concentrations of 0 (□), 3 (▴), 30 (■), 300 (♦), or 1200 nm (●) corresponded to CS/LAP-A ratios of 1:0.01, 1:0.1, 1:1, and 1:4, respectively. Neither His₆-LAP-A (○) nor lysozyme (△) aggregated on their own. B, CS was incubated with 900 nm His₆-LAP-A (■) or His₆-LAP-N (▴). His₆-LAP-N did not aggregate on its own (△). Aggregation of CS was determined by measuring light scattering at 360 nm. After 60 min at 43 °C, aggregation of 300 nm CS reached an absorbance of 0.8–1. Data shown are representative of two or more independent experiments. His₆-LAP-A (900 nm) reduction of CS aggregation varied in independent assays from 40 to 60%.