FIGURE 6.

LAP-A mutants aid refolding of Luc. A, Luc (1 μm) was heated for 11 min at 42 °C with 3 μm His₆-LAP-A (■); with one of the non-disruption mutants E429V (♦), D347N (▴), K354R (●), or R431A (×); or alone (no chap, □). B, Luc (1 μm) was heated for 11 min with 3 μm His₆-LAP-A (■); with one of the disruption mutants E429R (♦), D347R (▴), or K354E (●); or alone (no chap, □). Luc was allowed to refold, and its activity was measured as described in the legend to Fig. 3. Data shown are representative of at least two independent experiments.