FIGURE 8.

Simultaneous ligation of CD40 and α5β1 with specific antibodies synergizes in MMP-2 and MMP-9 secretion in U937 cells. CD40-transfected U937 cells were analyzed at base line (medium) or upon stimulation with anti-CD40 (G28.5) or anti-α5β1 (JBS5), alone or in combination, for 48 h at 37 °C. 45 μl of the different supernatants were analyzed at 4 °C on an 8% SDS-polyacrylamide gel containing 2 mg/ml gelatin. Gelatin zymography of MMP-2 and MMP-9 is represented in A. Densitometry measurements of MMP-2 and MMP-9 secreted by U937 cells are represented in B and C, respectively. This figure is representative of three independent experiments.