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. 2012 Mar 29;287(22):18055–18066. doi: 10.1074/jbc.M111.333989

TABLE 1.

Primers for cloning CD154 wild type and mutants

Sequence name Primer sequence
sCD154wt Forward, 5′-C GGG AGA TCT ATG CAA AAA GGT GAT-3′
Reverse, 5′-TAG ACT CGA GTT TGA GTA AGC C- 3′
sCD154-D117E Forward, 5′-C GGG AGA TCT ATG CAA AAA GGT GAA-3′
Reverse, 5′-TAG ACT CGA GTT TGA GTA AGC C-3′
sCD154-R203A Forward, 5′-GAG TAA GAT TGC CTC GAA TCT-3′
Reverse, 5′-AGA TTC GAG GCA ATC TTA CTC-3′
sCD154-Y145A Forward, 5′-GAA AAA GGA GCC TAC ACC ATG-3′
Reverse, 5′-CAT GGT GTA GGC TCC TTT TTC-3′