TABLE 1.
Sequence name | Primer sequence |
---|---|
sCD154wt | Forward, 5′-C GGG AGA TCT ATG CAA AAA GGT GAT-3′ |
Reverse, 5′-TAG ACT CGA GTT TGA GTA AGC C- 3′ | |
sCD154-D117E | Forward, 5′-C GGG AGA TCT ATG CAA AAA GGT GAA-3′ |
Reverse, 5′-TAG ACT CGA GTT TGA GTA AGC C-3′ | |
sCD154-R203A | Forward, 5′-GAG TAA GAT TGC CTC GAA TCT-3′ |
Reverse, 5′-AGA TTC GAG GCA ATC TTA CTC-3′ | |
sCD154-Y145A | Forward, 5′-GAA AAA GGA GCC TAC ACC ATG-3′ |
Reverse, 5′-CAT GGT GTA GGC TCC TTT TTC-3′ |