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. 2012 Apr 9;287(22):18091–18102. doi: 10.1074/jbc.M111.313163

FIGURE 5.

FIGURE 5.

ErbB4 participates in stretch-induced ERK phosphorylation and type II cell differentiation. A, E17 type II cells were transfected with scramble siRNA or siRNA for ErbB4; 2 days later, monolayers were exposed to 5% stretch for 15 min. The level of ERK activation was evaluated by Western blot. Upper panel shows a representative blot. n = 3; *, p < 0.01 versus non-stretch scramble, **, p < 0.05 versus stretch scramble. B, fetal type II cells were transfected with scramble siRNA, siRNA ErbB4 or siRNA ErbB4 plus active MEK; 24 h after transfection, cells were exposed to 5% stretch for 16 h. SP-C mRNA abundance was assessed by qRT-PCR. n = 3; *, p < 0.01 versus non-stretch scramble, **, p < 0.01 versus non-stretch siRNA ErbB4, ***, p < 0.05 versus non-stretch scramble. C, fetal type II cells were transfected as indicated; 2 days later, monolayers were fixed and stained by fluorescence immunocytochemistry to detect SP-C protein (red). Pictures are representative of three separate experiments. Bar, 100 μm.