FIGURE 5.

Measurements of distance between O6 from the target base (G or oxoG) and exocyclic atom at C6 (A and G) or C4 (T and C) position of the 5′-neighbor. Distance from the 5′-neighbor to the N4 of the opposing C is also shown. The target base and its base-stacking partner are consistently closer in distance in the G-bound state compared with the oxoG-bound state. Additionally, alignments between the oxoG-bound and G-bound states show how the position of the target oxoG responds to changes in the backbone conformation. Rotation of the backbone away from the state seen in the G-bound structure pulls the target oxoG into a more planar position, reducing the buckle angle of the target oxoG base pair compared with the buckle for the target G base pair.