FIGURE 6.

CANT1 modifies the secretion efficiency of the soluble folding-competent substrate wild type α1-antitrypsin (α1AT-WT). A, radiolabeled α1AT-WT was immunoisolated at the indicated times after the pulse from detergent extract (Intracellular, IC) and from the medium (Secreted, SE) of control cells (Ctr) or wtDREAM and daDREAM expressing clones. B, same as A, but in the presence of CANT1 overexpression. Quantification of secretion kinetics from two independent experiments in duplicate is shown as mean % ± S.E. for each panel. C and D show histograms of the average % values of secreted α1AT-WT at 45 and 90 min chase time. ns, not significant; ***, p < 0.001.