FIGURE 7.

CANT1 intracellular level affects ER-associated degradation of the soluble folding-defective substrate α1AT-NHK variant. A, radiolabeled α1AT-NHK was immunoisolated after the indicated chase times from detergent extract of control cells (Ctr) or wtDREAM- and daDREAM-expressing clones. B, same as in A but in the presence of overexpressed CANT1. C, quantification of intracellular α1AT-NHK degradation for each cell clone is shown in the absence or in the presence of overexpressed CANT1. D and E show histograms of the average % values at 10, 60, and 120 min chase time. F, Western blot of CANT1 silencing in neuroblastoma cells. G, quantification of CANT1 reduction in selected clonal population. H, radiolabeled α1AT-NHK was immunoisolated after the indicated chase times from detergent extract of shRNA control cells or shRNA CANT1-transfected cells. I, quantification of intracellular α1AT-NHK degradation for clonal population having reduced CANT1 levels is shown. I, shows histograms of the average % values at 10, 60, and 120 min chase time. Data are the mean % ± S.E. of three independent experiments in duplicate. ns, not significant; *, p < 0.05; **, p < 0.01.