FIGURE 2.

Stabilization of p53 in IAV-infected cells. A, Vero cells were mock-infected (mock) or infected with influenza A/Swine/Jiangsu/2/2006 (IAV) at a MOI of 5. The cells were first mock-treated (MG132− panels) or treated with 10 μm of MG132 (MG132+ panels) at 12 h postinfection (hpi) and subsequently exposed to 100 μg/ml of CHX at 16 hpi. The cells were collected at the indicated times (hours) post-treatment (hpt) and subjected to Western blot analysis using the indicated antibodies. B, change in abundance of p53 after CHX treatment in MG132-untreated cells (MG132− panels) was determined by densitometric analysis and normalized to β-actin. The relative abundance of p53 at each hpt was plotted. Results are presented as the means ± S.E. from three independent experiments. The black and gray bars indicate the IAV- and mock-infected cells, respectively.