FIGURE 3.

Detection of p53 ubiquitination and p300 in IAV-infected cells. Vero and CV-1 cells were mock-infected (mock) or infected with influenza A/Swine/Jiangsu/2/2006 (IAV) at a MOI of 5. A, cells were treated with 10 μm of MG132 or mock-treated at 12 h postinfection. The cell lysates were prepared 6 h after MG132 treatment and subjected to Western blot analysis using the indicated antibodies. To detect the ladders of polyubiquitinated p53 (Ub-p53), films were exposed ∼30 min longer than for the short-exposure p53 films. B, cells were treated with 10 μm TIBC alone, 10 μm MG132 alone, or a combination of both, or mock-treated, at 12 h postinfection. The cell lysates were prepared 6 h after treatment and subjected to Western blot analysis using the indicated antibodies. C, CV-1 cells were collected at the indicated time points postinfection (hpi) and subjected to Western blot analysis using the indicated antibodies.