UV-visible spectra of purified D-L(His), D-L(His)-FeS, and DΔD3/L(His) heterodimers.
a, D-L(His) (67.5 μm); b, D-L(His)-FeS (13.5 μm); c, DΔD3/L(His) (67.5 μm). The inset shows the D-L(His)-FeS heterodimer before (solid line) and after (dashed line) the addition of sodium dithionite (10 mm). AU, absorbance units.