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. 2012 Mar 28;287(22):18510–18523. doi: 10.1074/jbc.M111.331199

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Co-purification of M. acetivorans subunit DΔD3 with subunit L(His) after co-expression in E. coli. A, anaerobic size exclusion chromatography of eluate (∼50 mg of total protein) from Ni²⁺ affinity chromatography of E. coli cell lysate containing M. acetivorans subunits L(His) and DΔD3. The asterisk indicates the peak that is consistent with a the molecular mass of a heterodimer of DΔD3-L(His) (34.8 kDa). B, SDS-PAGE analysis of the purified D-L(His) (lane 1) and DΔD3-L(His) (lane 2) heterodimers, confirming the smaller molecular mass of DΔD3 (23.3 kDa) compared with D (28.7 kDa). mAU, milliabsorbance units.