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Effect of oxygen on stability of the [4Fe-4S] clusters in purified D-L(His)-FeS heterodimer. The loss of the [4Fe-4S] clusters in purified D-L(His)-FeS (20 μm) incubated under N2 or in air in 50 mm HEPES, 50 mm NaCl (pH 7.5) was monitored by measuring A390 over time. AU, absorbance units.