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. 2012 Mar 28;287(22):18510–18523. doi: 10.1074/jbc.M111.331199

FIGURE 8.

FIGURE 8.

Effect of oxygen on stability of D-L(His), D-L(His)-FeS, and DΔD3-L(His) heterodimers. Each heterodimer was incubated anaerobically or aerobically in 50 mm HEPES, 50 mm NaCl (pH 7.5) and analyzed by size exclusion chromatography under anaerobic conditions using a running buffer of 50 mm HEPES (pH 7.5), 150 mm NaCl. Elution profiles of each heterodimer after anaerobic (solid line) or aerobic incubation (dashed line) are shown in A–C. A, D-L(His), 1.1 mg of each; B, D-L(His)-FeS, 0.8 mg of each; C, DΔD3/L(His), 1.5 mg of each. For each elution profile, an asterisk indicates the heterodimer peak, and arrows indicate D-L(His) aggregates. The UV-visible spectra of concentrated fractions containing heterodimer from anaerobic samples (solid line) and aerobic samples (dashed line) are shown in D–F. D, D-L(His), 26 μm each; E, D-L(His)-FeS, 10 μm each; F, DΔD3-L(His),18 μm each. AU, absorbance units; mAU, milliabsorbance units.