JNK pathway mediates osmotic stress-induced Raptor phosphorylation.
A, HEK293 cells were serum-starved overnight, pretreated with 20 μm SP600125, 10 μm U0126, 10 μm SB203580, 10 μm Roscovitine, 20 nm rapamycin, or 10 μm Compound C for 30 min, and then exposed to 0.5 m sorbitol for an additional 60 min. The lysates were immunoblotted with the indicated antibodies. p-Raptor, phospho-Raptor. B, HEK293 cells transfected with control or JNK1/2 siRNA were serum-starved overnight and exposed to 0.5 m sorbitol for 60 min. The lysates were immunoblotted with the indicated antibodies.