FIGURE 2.

Attenuation of Src decreases LPS-mediated Eps8 expression and macrophage phagocytosis. RAW cells and their derived Ctrl and siRNA-1 cells as well as siRNA-1 cells harboring a plasmid encoding avian Src (siRNA-1/Src6 and siRNA-1/Src15) were stimulated with or without LPS (100 ng/ml) for 48 h. Then either these cells were lysed and their lysates were Western immunoblotted with antibodies as indicated (A), or they were incubated with GFP-E. coli (B) or latex beads (C) at 37 °C for 1 h. The uptake of E. coli by macrophages was assayed by FACS analysis (B), whereas the uptake of latex beads by macrophages was determined by a fluorescence microscope (C). The phagocytosis index was calculated as the number of ingested beads in 100 cells. The experiment was performed at least three times in triplicate. The results were means ± S.E. ***, p < 0.001.