FIGURE 5.

Attenuation of FAK inhibits LPS-induced Eps8 expression and the uptake of GFP-E. coli in RAW264.7 cells. Generation of RAW stably expressing nonspecific siRNA (Ctrl) and two different fak siRNAs (fak siRNA-1 and -2) cells was described previously (27). These cells were stimulated without (−) or with LPS (100 ng/ml) for 24 h. The cell lysates (100 μg) were Western immunoblotted with antibodies as indicated (top), and the amounts of fak, eps8, and src transcript were analyzed by RT-PCR (middle). gapdh was utilized as an internal control for amplification efficiency. In a parallel experiment, these cells were incubated with GFP-E. coli at 37 °C for 1 h, followed by FACS analysis to determine their ability to uptake E. coli (bottom).