FIGURE 2.

Response of white adipocytes to hypoxia requires HIF-1α. 3T3-L1 preadipocytes were differentiated until day 8, at which time cells were exposed to DeliverX siRNA reagent alone (C, Con) or with oligonucleotides specific to HIF-1α (KD). 48 h later, cells were exposed to 20% or 1% O₂ for 18 h. The cells were immediately harvested in lysis buffer for protein isolation and Western blot analysis (A) or in TRIzol reagent for RNA isolation and quantitative real-time PCR analysis (B). Results are means ± S.E. (n = 3).