FIGURE 4.

TZD suppresses hypoxia-associated induction of inflammatory genes and enhances expression of HIF-1α targets. 3T3-L1 preadipocytes were differentiated until day 8 with either 5 μm TZD or Me₂SO vehicle added to the medium. On day 8, cells were exposed to 20% or 1% O₂ for 18 h. The cells were immediately harvested in TRIzol reagent for RNA isolation and quantitative real-time PCR analysis for HIF-1 targets (A) or inflammatory genes (B). Results are means ± S.E. (n = 3). *, p = < 0.01; +, p < 0.05.