FIGURE 5.

PPARγ deficiency attenuates induction of hypoxia-responsive genes while increasing expression of inflammatory genes in mature hypoxic adipocytes. 3T3-L1 preadipocytes were differentiated until day 4, and then either 5 μm TZD or Me₂SO vehicle was added to the medium until day 10. On day 8, cells were exposed to DeliverX siRNA reagent alone or with oligonucleotides specific to PPARγ. On day 9, cells were exposed to 20% or 1% O₂ for 18 h. The cells were immediately harvested in lysis buffer for protein isolation and Western blot analysis (A) or in TRIzol reagent for RNA isolation and quantitative real-time PCR analysis (B and C). Results are means ± S.E. (n = 3). siPPARγ, PPARγ siRNA.