FIGURE 3.

Ty3 IN interacts with target DNA via interactions mediated by TFP. Protein-³²P-labeled DNA complexes were formed with 0.3 nm 57-bp TATA box-containing probe (see “Experimental Procedures”: EMSA), 12.5 nm TFP (middle and right panels), and increasing amounts of IN (0, 12.5, 25, 50 nm), and were analyzed on a 4% native polyacrylamide gel. Binding buffers contained 7 mm MgCl₂ (all panels), and MnCl₂-containing reactions were 10 mm (right panel). IN was titrated in each series (− and wedge).