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. 2012 Apr 21;2(3):330–334.

Figure 1.

Figure 1

Myc activates SAE1 expression. (A) mRNA expression levels of SAE1, NCL and PUMA were quantified by qRT–PCR in quiescent cells (0) and after 4 and 6hrs of treatment with serum and OHT. (B) Myc expression was inhibited with specific siRNA (siMyc) and siRNA non-targeting (siCtl) was used as scrambled RNAs. SAE1 mRNAs expression levels were quantified by qRT–PCR in quiescent cells (0) and 6hrs of treatment with serum and OHT. The efficiency of Myc silencing by siRNA treatments measured by qRT–PCR is shown on the right. (C) RAT-Myc-/- cells as well as cells transfected with a Myc expression vector were serum deprived for 48 hrs. SAE1 mRNA levels were evaluated by qRT– PCR 6 hrs after serum induction. Values were compared to quiescent cells (0).