Figure 4.

CsA regulates the trophoblasts invasiveness through improving the cross-talk between DSCs and trophoblasts via CXCL12 and CD82. The CD82-silenced DSCs (1×10⁵cells/well) in the lower and or trophoblast cells (1×10⁵cells/well) in the upper chamber were pretreated with or without anti-CXCR4 neutralizing antibody (4ug/ml) for 4h, then was formed a indirect co-culture unit and treated with or without CsA (1uM). Subsequently, Matrigel invasion assay was used to detect the invasiveness of trophoblasts in co-culture unit. Silence: CD82 was knocked down. DSCs (α-CXCR4): DSCs were pretreated with anti-CXCR4 neutralizing antibody; Tro (α-CXCR4): Trophoblasts were pretreated with anti-CXCR4 neutralizing antibody. Results were highly reproducible in three independent experiments. Error bars depict the standard error of the mean. *P<0.05, **P<0.01 compared to nontargeting siRNA control. #P<0.05 compared to nontargeting siRNA control and anti-CXCR4 neutralizing antibody pretreated DSCs. ΔP<0.05 compared to compared to nontargeting siRNA control and anti-CXCR4 neutralizing antibody pretreated trophoblasts.