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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Methods Mol Biol. 2011;716:89–126. doi: 10.1007/978-1-61779-012-6_6

Fig. 6.

Fig. 6

Detection of urokinase plasminogen activator (uPA) with 7. (a) The proposed mechanism of uPA-mediated cleavage of the Z-GGR peptide ligand from the agent and showing conversion of an amide proton to an amine. (b) The MTRasy spectrum showed a CEST effect from the amide at −52 ppm before uPA was added (red). The disappearance of this CEST effect after uPA was added (blue) was used to detect uPA. An enzyme-unresponsive agent, Yb-DOTA-Gly, showed a CEST effect at −16 ppm before and after uPA was added, which served as an internal control.