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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Methods Mol Biol. 2011;716:89–126. doi: 10.1007/978-1-61779-012-6_6

Table 1.

HPLC conditions used for various compounds

Phase A Phase B
A1: 0.1% TFA in water B1: 0.1% TFA in acetonitrile
A2: 0.1% TEA/AcOH (TEAA) in water (pH 6.0) B2: 90% acetonitrile in Phase A2 (pH adjusted to 6.0 with AcOH)

Method A: 10–40% Phase B1 in Phase A1 in 30 min; Method B: 10–60% Phase B1 in Phase A1 in 50 min; Method C: 10–90% Phase B1 in Phase A1 in 30 min; Method D: 20–60% Phase B1 in Phase A1 in 50 min; Method E: 10–60% Phase B2 in Phase A2 in 50 min; Method F: 10–90% Phase B2 in Phase A2 in 40 min