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Triple label of recorded GnRH neuron from a GnRH-GFP transgenic mouse. A, GFP signal used to target recording pipette to a GnRH neuron. B, Biocytin labeling of recorded cell detected with streptavidin-Cy3. C, Immunostaining for GnRH with Cy5-conjugated secondary antibody. [Data adapted from K. J. Suter et al.: Endocrinology 141:412–419 (73). © 2000, The Endocrine Society].
