Figure 5.

Pharmacological rescue of six intracellularly retained MC4R mutants by ML00253764. Human embryonic kidney 293 cells stably transfected with WT or mutant MC4Rs or empty vector pcDNA3.1 (as a negative control) were incubated with 10 μm ML00253764 for 24 h at 37 C. Cells were then stained with anti-myc monoclonal antibody to visualize the c-myc epitope at the N terminus of the MC4R by confocal microscopy. Detailed procedures are described in Ref. 346.