Figure 2.

Gene deletion of RAGE or Myd88 affects proliferation after extensive hepatectomy. Male C57BL/6 mice were subjected to 85% hepatectomy or sham surgery. A) At the indicated times, hepatic remnants were subjected to immunoblotting with anti-cleaved caspase 3 IgG. Illustrated bands are representative of n = 3–4 mice/condition. B) mRNA transcript levels for Bax were determined in the indicated mice by real time PCR. C) Pcna staining. Hepatic remnants were retrieved and subjected to immunohistochemistry with anti-Pcna IgG (×20 view). Arrows indicate Pcna⁺ cells. Data are representative of n = 4 mice/condition. D) mRNA transcript levels for cyclin D1 were determined in the liver remnants of the indicated mice by real-time PCR. Data are means ± se; n = 4. *P < 0.05.