Figure 3.

Gene deletion of RAGE modulates Glo1, MG, and AGE production, and NF-κB activation. A) mRNA transcript levels for Glo1 in the liver remnants were determined in the indicated mice by real-time PCR. Data are means ± se; n = 5. B) Equal amounts of protein extracts from hepatic remnants were subjected to immunoblotting with anti-Glo1 IgG. Illustrated bands are representative of n = 4 mice/condition. C) Glo1 activity was assessed as described in the indicated groups; n = 4 mice/condition. D, E) At the indicated times, MG (D) and AGEs (E) were determined in the liver remnants as described; n = 4 mice/condition. F) WT and RAGE-null remnants were retrieved 2 h after extensive hepatectomy. ChIP was performed using anti-NF-κB p65 IgG, and the ChIP-enriched DNA was subjected to PCR for Glo1; n = 3 mice/condition. G) Nuclear extracts were prepared from the remnants of the indicated mice, and EMSA for NF-κB was performed; n = 4 mice/condition. H) mRNA transcript levels for p65 in the liver remnants were determined in the indicated mice by real-time PCR; n = 4–5. I) mRNA transcript levels for TNF-α in the liver remnants were determined in the indicated mice by real-time PCR; n = 4–5. Data are means ± se. *P < 0.05.